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induro reverse transcriptase  (New England Biolabs)


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    Structured Review

    New England Biolabs induro reverse transcriptase
    Induro Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase/Induro+Reverse+Transcriptase/bio_rxiv__64898__2026__10__14__759216-312-24-27
    Average 98 stars, based on 142 article reviews
    induro reverse transcriptase - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Assessing the integrity of SARS-CoV-2 and F-specific RNA bacteriophage RNA in raw wastewater (ANRS0160).
    Article Snippet: .. The reverse transcriptase selected for the study was the Induro® Reverse Transcriptase (NEB Biolabs, M0681S) that generates complementary DNA of up to 12,000 nt in length according to the manufacturer’s specifications. ..

    Article Title: Proteins, Processing, and Properties of Adhesive Fluid Condensates Purified from Mussels
    Article Snippet: [20] The database search results were loaded onto Scaffold version 5.1.1 (Proteome Sciences) for statistical treatment and data visualization. .. Rapid Amplification of cDNA Ends (RACE): 5′ and 3′ RACE libraries were prepared from plaque gland total RNA using a template switching reverse transcriptase (NEB #M0466) and the following oligos to prime cDNA synthesis: for 3′ RACE 5′-AAGCAGTGGTATCAACGCAGAGTACT7GT9CT10VN3′; for 5′ RACE an oligo dT primer (5′-T25VN-3′) and 5′- AAGCAGTGGTATCAACGCAGAGTACGCrG*rG*rG*rG*rG*-3′ where the 5 guanine residues at the 3′ end of this primer (“rG*”) are ribo-nucleotides. ..

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces.
    Article Snippet: .. The two enzymes utilized in the RT-LAMP assay include a strand-displacing DNA polymerase (New England Biolabs Bst 2.0 WarmStart® DNA Polymerase), which has a manufacturer-reported optimal reaction performance of 60 ◦C to 72 ◦C, and a reverse transcriptase (New England Biolabs WarmStart® RTx Reverse Transcriptase), which has a reported optimal reaction performance of 55 ◦C to 65 ◦C, with the overlap in temperature range (65 ◦C) representing the optimum incubation temperature for the RT-LAMP assay. ..

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
    Article Snippet: .. The two enzymes utilized in the RT-LAMP assay include a strand-displacing DNA polymerase (New England Biolabs Bst 2.0 WarmStart ® DNA Polymerase), which has a manufacturer-reported optimal reaction performance of 60 °C to 72 °C, and a reverse transcriptase (New England Biolabs WarmStart ® RTx Reverse Transcriptase), which has a reported optimal reaction performance of 55 °C to 65 °C, with the overlap in temperature range (65 °C) representing the optimum incubation temperature for the RT-LAMP assay. ..

    Article Title: Extended analyses of rotavirus C (RVC) G-types and P-types reveal new cut-off value for the G-types and reclassification of strains
    Article Snippet: A mixture of primers (10 mM), dNTPs (10 mM), nuclease-free water, and RNA was heated to 97°C for 5 min and chilled on ice immediately for at least 1 min. A master mix containing buffer, reverse transcriptase (100 U), DTT (0.1 M), and Ribolock RNAse inhibitor (40 U) was subsequently added. .. RT was carried out at 55°C for 60 min, followed by heat inactivation of the reverse transcriptase at 70°C for 15 min. PCR was conducted using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, Frankfurt, Germany) according to the manufacturer’s instructions. ..

    Article Title: Massively parallel COVID-19 diagnostic assay for simultaneous testing of 19200 patient samples
    Article Snippet: .. Any reverse transcriptase may be used with the present methods including, without limitation, avian myeloblastosis virus (AMV) reverse transcriptase, Moloney murine leukemia virus (M-MuLV) reverse transcriptase, SuperscriptTM III Reverse Transcriptase (ThermoFisher), and ProtoScript® II Reverse Transcriptase (NEB). .. In the Examples, the inventors utilized ProtoScript® II Reverse Transcriptase (NEB), which is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity.

    other:

    Article Title: A direct RNA-seq-based EBV latency transcriptome offers insights into the biogenesis of EBV gene products.
    Article Snippet: This could be reduced by using a reverse transcriptase with better processivity, such as Induro (NEB), which improves the proportion of intact 5′ ends of transcripts [65], and is now recommended by 12 Mamane- Logsdon et al., Journal of General Virology 2025;106:002134 Oxford Nanopore Technologies.

    Article Title: A direct RNA-seq-based EBV Latency Transcriptome Offers Insights into the Biogenesis of EBV Gene Products
    Article Snippet: This could be reduced by using a reverse transcriptase with better processivity, such as Induro (NEB), which improves the proportion of intact 5’ ends of transcripts [ ], and is now recommended by Oxford Nanopore Technologies.

    Rapid Amplification of cDNA Ends:

    Article Title: Proteins, Processing, and Properties of Adhesive Fluid Condensates Purified from Mussels
    Article Snippet: [20] The database search results were loaded onto Scaffold version 5.1.1 (Proteome Sciences) for statistical treatment and data visualization. .. Rapid Amplification of cDNA Ends (RACE): 5′ and 3′ RACE libraries were prepared from plaque gland total RNA using a template switching reverse transcriptase (NEB #M0466) and the following oligos to prime cDNA synthesis: for 3′ RACE 5′-AAGCAGTGGTATCAACGCAGAGTACT7GT9CT10VN3′; for 5′ RACE an oligo dT primer (5′-T25VN-3′) and 5′- AAGCAGTGGTATCAACGCAGAGTACGCrG*rG*rG*rG*rG*-3′ where the 5 guanine residues at the 3′ end of this primer (“rG*”) are ribo-nucleotides. ..

    cDNA Synthesis:

    Article Title: Proteins, Processing, and Properties of Adhesive Fluid Condensates Purified from Mussels
    Article Snippet: [20] The database search results were loaded onto Scaffold version 5.1.1 (Proteome Sciences) for statistical treatment and data visualization. .. Rapid Amplification of cDNA Ends (RACE): 5′ and 3′ RACE libraries were prepared from plaque gland total RNA using a template switching reverse transcriptase (NEB #M0466) and the following oligos to prime cDNA synthesis: for 3′ RACE 5′-AAGCAGTGGTATCAACGCAGAGTACT7GT9CT10VN3′; for 5′ RACE an oligo dT primer (5′-T25VN-3′) and 5′- AAGCAGTGGTATCAACGCAGAGTACGCrG*rG*rG*rG*rG*-3′ where the 5 guanine residues at the 3′ end of this primer (“rG*”) are ribo-nucleotides. ..

    RT Lamp Assay:

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces.
    Article Snippet: .. The two enzymes utilized in the RT-LAMP assay include a strand-displacing DNA polymerase (New England Biolabs Bst 2.0 WarmStart® DNA Polymerase), which has a manufacturer-reported optimal reaction performance of 60 ◦C to 72 ◦C, and a reverse transcriptase (New England Biolabs WarmStart® RTx Reverse Transcriptase), which has a reported optimal reaction performance of 55 ◦C to 65 ◦C, with the overlap in temperature range (65 ◦C) representing the optimum incubation temperature for the RT-LAMP assay. ..

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
    Article Snippet: .. The two enzymes utilized in the RT-LAMP assay include a strand-displacing DNA polymerase (New England Biolabs Bst 2.0 WarmStart ® DNA Polymerase), which has a manufacturer-reported optimal reaction performance of 60 °C to 72 °C, and a reverse transcriptase (New England Biolabs WarmStart ® RTx Reverse Transcriptase), which has a reported optimal reaction performance of 55 °C to 65 °C, with the overlap in temperature range (65 °C) representing the optimum incubation temperature for the RT-LAMP assay. ..

    Incubation:

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces.
    Article Snippet: .. The two enzymes utilized in the RT-LAMP assay include a strand-displacing DNA polymerase (New England Biolabs Bst 2.0 WarmStart® DNA Polymerase), which has a manufacturer-reported optimal reaction performance of 60 ◦C to 72 ◦C, and a reverse transcriptase (New England Biolabs WarmStart® RTx Reverse Transcriptase), which has a reported optimal reaction performance of 55 ◦C to 65 ◦C, with the overlap in temperature range (65 ◦C) representing the optimum incubation temperature for the RT-LAMP assay. ..

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
    Article Snippet: .. The two enzymes utilized in the RT-LAMP assay include a strand-displacing DNA polymerase (New England Biolabs Bst 2.0 WarmStart ® DNA Polymerase), which has a manufacturer-reported optimal reaction performance of 60 °C to 72 °C, and a reverse transcriptase (New England Biolabs WarmStart ® RTx Reverse Transcriptase), which has a reported optimal reaction performance of 55 °C to 65 °C, with the overlap in temperature range (65 °C) representing the optimum incubation temperature for the RT-LAMP assay. ..

    Inactivation:

    Article Title: Extended analyses of rotavirus C (RVC) G-types and P-types reveal new cut-off value for the G-types and reclassification of strains
    Article Snippet: A mixture of primers (10 mM), dNTPs (10 mM), nuclease-free water, and RNA was heated to 97°C for 5 min and chilled on ice immediately for at least 1 min. A master mix containing buffer, reverse transcriptase (100 U), DTT (0.1 M), and Ribolock RNAse inhibitor (40 U) was subsequently added. .. RT was carried out at 55°C for 60 min, followed by heat inactivation of the reverse transcriptase at 70°C for 15 min. PCR was conducted using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, Frankfurt, Germany) according to the manufacturer’s instructions. ..

    Polymerase Chain Reaction:

    Article Title: Extended analyses of rotavirus C (RVC) G-types and P-types reveal new cut-off value for the G-types and reclassification of strains
    Article Snippet: A mixture of primers (10 mM), dNTPs (10 mM), nuclease-free water, and RNA was heated to 97°C for 5 min and chilled on ice immediately for at least 1 min. A master mix containing buffer, reverse transcriptase (100 U), DTT (0.1 M), and Ribolock RNAse inhibitor (40 U) was subsequently added. .. RT was carried out at 55°C for 60 min, followed by heat inactivation of the reverse transcriptase at 70°C for 15 min. PCR was conducted using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, Frankfurt, Germany) according to the manufacturer’s instructions. ..

    Virus:

    Article Title: Massively parallel COVID-19 diagnostic assay for simultaneous testing of 19200 patient samples
    Article Snippet: .. Any reverse transcriptase may be used with the present methods including, without limitation, avian myeloblastosis virus (AMV) reverse transcriptase, Moloney murine leukemia virus (M-MuLV) reverse transcriptase, SuperscriptTM III Reverse Transcriptase (ThermoFisher), and ProtoScript® II Reverse Transcriptase (NEB). .. In the Examples, the inventors utilized ProtoScript® II Reverse Transcriptase (NEB), which is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity.



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